HPLC determination of insulin and its related substances in insulin powder for inhalation / 药学学报
Acta Pharmaceutica Sinica
;
(12): 131-133, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-343384
ABSTRACT
<p><b>AIM</b>To determine insulin and its related substances in insulin powder for inhalation by reversed phase high performance liquid chromatography method.</p><p><b>METHODS</b>The initial mobile phase was solution A (0.1% trifluoroacetic acid-acetonitril 7030) and changed to solution B (0.1% trifluoroacetic acid-acetonitril 6040) in 30 minutes. The flow rate was 2.0 mL.min-1, the column temperature was 30 degrees C, the wave length was 280 nm, the injected volume was 20 microL.</p><p><b>RESULTS</b>Insulin was well separated from other peaks induced in different conditions. There was good linear relationship between the amount of insulin and its peak area, the RSD was 1.1%, the insulin solution for determination was stable in 12 hours, and the quantity detected was near the added.</p><p><b>CONCLUSION</b>The method is simple and accurate to detect insulin and its related substances in insulin and its preparations.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Polvos
/
Administración por Inhalación
/
Cromatografía Líquida de Alta Presión
/
Insulina
/
Métodos
Idioma:
Chino
Revista:
Acta Pharmaceutica Sinica
Año:
2002
Tipo del documento:
Artículo
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