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Induction of CuZn-SOD mRNA expression and activity by PGMS in rat liver / 药学学报
Acta Pharmaceutica Sinica ; (12): 23-26, 2002.
Artículo en Chino | WPRIM | ID: wpr-343408
ABSTRACT
<p><b>AIM</b>To study the effect of propylene glycol mannate sulfate (PGMS) on induction of CuZn-SOD.</p><p><b>METHODS</b>Wistar rats were given PGMS p.o. at different doses (0, 18.9, 37.8 and 75.6 mg.kg-1.d) for ten days. Then the rats were sacrificed and the total RNA was extracted from the livers. The total RNA samples were loaded on a 1% agarose gel to detect the quality of total RNA. RT-PCR was applied to study the expression of CuZn-SOD mRNA in rat livers. The amplified products were detected by the 1.5% agarose gel electrophoresis. Simultaneously, the CuZn-SOD activities in rat liver were determined by nitrite method.</p><p><b>RESULTS</b>The total RNA extracted from rat livers was integrated without being decomposed by RNase. The level of CuZn-SOD mRNA of the high-dosage group (75.6 mg.kg-1.d) was higher than that of the control group (0 mg.kg-1.d) (P < 0.01); the CuZn-SOD activities of the high-dosage group were significantly higher than those of the control group (P < 0.001) and the CuZn-SOD activities of the middle- (37.8 mg.kg-1.d) and low-dosage groups (18.9 mg.kg-1.d) were higher than those of the control group (P < 0.01).</p><p><b>CONCLUSION</b>PGMS can increase the CuZn-SOD activities as well as CuZn-SOD on mRNA level. Therefore, it is possible for PGMS to counteract Atherosclerosis (AS) by inducing the expression of CuZn-SOD.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Glicoles de Propileno / Superóxido Dismutasa / Técnicas In Vitro / ARN Mensajero / Depuradores de Radicales Libres / Ratas Wistar / Relación Dosis-Respuesta a Droga / Genética / Hígado Límite: Animales Idioma: Chino Revista: Acta Pharmaceutica Sinica Año: 2002 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Glicoles de Propileno / Superóxido Dismutasa / Técnicas In Vitro / ARN Mensajero / Depuradores de Radicales Libres / Ratas Wistar / Relación Dosis-Respuesta a Droga / Genética / Hígado Límite: Animales Idioma: Chino Revista: Acta Pharmaceutica Sinica Año: 2002 Tipo del documento: Artículo