Comparison and evaluation of two methods for measuring acid phosphatase activity in seminal plasma / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To compare the routine method and the kit method for the measurement of acid phosphatase activity in seminal plasma, and to explore the possibility of the kit method for routine measurement.</p><p><b>METHODS</b>Seventy-nine seminal plasma samples were assayed by routine method and kit method respectively for acid phosphatase. One sample was detected 10 times for within-run analysis, and an other two were measured by both the methods once a day for 10 days for between-run analysis. Acid phosphatase activities in another 10 seminal plasma samples collected at random were measured immediately or 30 min after dilution by two technicians, respectively.</p><p><b>RESULTS</b>There were significant positive correlations between the acid phosphatase activities measured by routine and kit methods (r = 0.745, P = 0.000). In the within-run assay, the coefficient of variation for the kit method (13.72%) was similar with that for the routine method (10.66%). But in the between-run assay, the coefficients of variation for the kit method (13.8% and 15.49%) were obviously lower than those for the routine method (24.43% and 21.04%). Compared with the acid phosphatase activities in seminal plasma measured immediately after dilution, those measured after 30-min standing were notably lower for either of the methods (P < 0.05). However, there wasnt significant difference in the acid phosphatase activities detected by the routine method between the two technicians (P = 0.165).</p><p><b>CONCLUSION</b>The kit method is superior and preferable to the routine method for the measurement of acid phosphatase in seminal plasma.</p>