Enhancement of herpes simplex virus-1 glycoprotein-D DNA vaccine induced specific immune responses by coimmunization with interleukin-2 genetic adjuvant / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 67-72, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-343765
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the immune responses and protection from virus challenge, induced by the coinjection of IL-2cDNA with herpes simplex virus type 1 (HSV-1) glycoprotein-D (gD) DNA vaccine.</p><p><b>METHODS</b>Two DNA vaccines (pgD and pIL-2) were constructed by inserting the gD gene and IL-2 cDNA into the eukaryotic expression vector pcDNA3.1, respectively. The BALB/c mice were inoculated intramuscularly three times at 2-week intervals. Two weeks after the final immunization, mice were bled for antibody assay and spleen cells were separated for Th cell proliferation and cytokine assays. Delayed type hypersensitivity (DTH) response was detected by the pinna-swelling test. Corneal protection under HSV-1 virus challenge was continuously observed with slit-lamp microscope.</p><p><b>RESULTS</b>IL-2 cDNA coinjection remarkably enhanced the specific IgG2a level when compared with gD plasmid vaccination alone. Th cell proliferation and secretion of cytokines (IL-2 and IFN-gamma) were significantly increased by IL-2 cDNA coinjection. However, the production of IL-10 was inhibited. The DTH response was also enhanced by IL-2 coinjection. When the mice were challenged with HSV-1, the cornea epithelial lesions were significantly alleviated by IL-2 coinjection as compared with gD vaccination alone.</p><p><b>CONCLUSION</b>IL-2 cDNA can enhance both the humoral and cellular immune responses, and thus increase the vaccine potency.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Virulencia
/
Sangre
/
ADN
/
Inmunoglobulina G
/
Vacunas Virales
/
Transfección
/
Distribución Aleatoria
/
Chlorocebus aethiops
/
Proteínas del Envoltorio Viral
/
Inmunización
Límite:
Animales
Idioma:
Chino
Revista:
Acta Academiae Medicinae Sinicae
Año:
2005
Tipo del documento:
Artículo
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