Palmitic acid induces apoptosis in human hepatoma cell line, HepG2 cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 671-676, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-343785
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of palmitic acid (PA) on human hepatocytes and its mechanism.</p><p><b>METHODS</b>We administered a mimic hyperlipidemia condition of 0.2-0.4 mmol/L PA to human hepatoma cell line, HepG2 cells. Cell viability was determined by Trypan blue staining. Cell cycle and early apoptosis were determined by propidium iodide and/or Annexin V staining, and the levels of Bcl-2 and Bax were analyzed by flow cytometry.</p><p><b>RESULTS</b>An inhibition of cell growth was observed at a dose- and time-dependent manner in HepG2 cells after the treatment of PA. An apoptosis with appearance of sub-G1 fraction determined by cell cycle analysis significantly increased after the treatment of PA for 4 days. Bcl-2 level slightly decreased; in contrast, Bax level elevated markedly, which resulted in a significant decrease of Bcl-2/Bax ratio.</p><p><b>CONCLUSION</b>PA may induce cell death on hepatocytes via mitochondria-mediated apoptosis by reducing the level of Bcl-2/Bax.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Ciclo Celular
/
Supervivencia Celular
/
Apoptosis
/
Carcinoma Hepatocelular
/
Ácido Palmítico
/
Proteínas Proto-Oncogénicas c-bcl-2
/
Línea Celular Tumoral
/
Proliferación Celular
Límite:
Humanos
Idioma:
Chino
Revista:
Acta Academiae Medicinae Sinicae
Año:
2004
Tipo del documento:
Artículo
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