Determination of ABO blood group genotypes with one tube PCR reaction / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1117-1119, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-343813
ABSTRACT
This study was aimed to establish one tube PCR reaction technique to determine ABO blood group genotypes. Salting-out method was adopted to extract genomic DNA; one tube polymerase chain reaction with GeneScan technique was used to identify ABO genotypes. The results showed that the ABO genotypes of 132 samples were in accordance with the phenotypes determined by serological technique. The frequencies of A, B and O were 0.205, 0.159 and 0.636 respectively. AA, AO, AB, BB, BO and OO genotypes were 8 (6.1%), 31 (23.5%), 7 (5.3%), 6 (4.5%), 23 (17.4%), and 57 (43.2%) respectively. It is concluded that one tube polymerase chain reaction with GeneScan technique can determine the genotypes of ABO blood group.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sistema del Grupo Sanguíneo ABO
/
Reacción en Cadena de la Polimerasa
/
Reproducibilidad de los Resultados
/
Frecuencia de los Genes
/
Genética
/
Genotipo
/
Métodos
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2005
Tipo del documento:
Artículo
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