A method for Rhesus box test / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1103-1105, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-343817
ABSTRACT
To study the method for Rhesus box test and its significance, the sequence specific primers of upstream, downstream and hybrid Rhesus boxes were designed according to RhD gene sequence; the upstream, downstream and hybrid Rhesus boxes were determined by PCP-SSP and mismatched PCR. The results showed that this method was confirmed by DNA Standard test. It was shown that in unrelative RhD positive individuals RHD(+)/RHD(-), RHD(+)/RHD(+) genotype accounted for 9.00%, 91.00% respectively, and in RhD negative individuals RHD(+)/RHD(-), RHD(+)/RHD(+), RHD(-)/RHD(-) genotype were 26.14%, 3.92%, 69.94% respectively. It is concluded that the method of Rhesus box test was confirmed to be reliable and can be used for the identification of RhD haplotype gene structure, as well as for study on inheritance, clinical transfusion and neonatal hemolytic diseases.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sistema del Grupo Sanguíneo Rh-Hr
/
Haplotipos
/
Secuencia de Bases
/
Reacción en Cadena de la Polimerasa
/
Genética
/
Heterocigoto
/
Homocigoto
/
Métodos
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2005
Tipo del documento:
Artículo
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