Establishment of beta block matching technique / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 901-903, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-343862
ABSTRACT
The purpose of this study was to establish beta block matching technique. DNA was extracted from whole blood by salting-out method, beta block matching was performed by PCR and GeneScan technique. The results showed that the length of fragments amplificated in 100 samples was different and the range of them was 91-197 bp. Amplification fragments could be divided into four regions 91-93, 105-113, 125-139 and 177-197 bp respectively. 91 bp DNA fragments could be found in all of samples. The numbers of DNA fragments with different length have been shown high polymorphism and they focused on the range of seven to twenty four. In conclusion, the beta block matching technique is reliable and applicable to the selection of hematopoietic stem cell transplantation donors.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ADN
/
Prueba de Histocompatibilidad
/
Antígenos HLA-B
/
Antígenos HLA-C
/
Antígenos HLA-DR
/
Reacción en Cadena de la Polimerasa
/
Trasplante de Células Madre Hematopoyéticas
/
Cadenas HLA-DRB1
/
Genética
/
Métodos
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2005
Tipo del documento:
Artículo
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