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Mechanism of differentiation and apoptosis in leukemia cells induced by tributyrin / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 774-777, 2005.
Artículo en Chino | WPRIM | ID: wpr-343889
ABSTRACT
To elucidate the possible mechanism of differentiation and/or apoptosis in NB4, K562 cells induced by tributyrin (TB), a histone deacetylase inhibitor (HDACi), the level of acetylated histone H3 was detected by Western blot, p21(WAF1) expression was detected by semi-quantitative RT-PCR. The results showed that histone H3 hyperacetylation was detected in NB4 (or K562) cells after treatment with TB 0.1 mmol/L (or TB 0.5 mmol/L) for 16 hours in a dose-dependent manner. p21(WAF1) dose-dependently increased at RNA level in these two cell lines treated by TB 0.1 mmol/L. The level of p21(WAF1) mRNA increased at 2 hours after TB treatment, reaching peak at 16 hours, and maintaining for 48 hours. In conclusion, the mechanism of differentiation and apoptosis in NB4, K562 cells induced by tributyrin may associate with its up-regulation of acetylated histone and p21(WAF1) mRNA level.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Farmacología / Acetilación / Triglicéridos / ARN Mensajero / Histonas / Leucemia / Regulación Neoplásica de la Expresión Génica / Diferenciación Celular / Western Blotting Límite: Humanos Idioma: Chino Revista: Journal of Experimental Hematology Año: 2005 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Farmacología / Acetilación / Triglicéridos / ARN Mensajero / Histonas / Leucemia / Regulación Neoplásica de la Expresión Génica / Diferenciación Celular / Western Blotting Límite: Humanos Idioma: Chino Revista: Journal of Experimental Hematology Año: 2005 Tipo del documento: Artículo