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Gene expression profile in K562 cells treated by interferon alpha / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 746-750, 2005.
Artículo en Chino | WPRIM | ID: wpr-343894
ABSTRACT
To study the gene expression profile in K562 cells treated by IFN-alpha, so as to provide some information about the potential mechanism of IFN-alpha curing CML, the changes of gene expression were examined with the DNA array in K562 cells before and after treatment with IFN-alpha. The results showed that no gene expression difference more than 2.5 times in K562 cells was found on the first day after treatment with IFN-alpha (200 U/ml), then the genes significant expression difference increased step by step, and reached the peak on the forth day. In all examined genes, 97 genes significant expression difference were detected, 86.60% (84/97) gene of interest out of those gene were up-regulated, 13.40% (13/97) were down-regulated. In these 97 genes with significant expression difference, cell regulator protein genes accounted to 23.71% (23/97), surface receptor genes 14.43% (14/97), oncogenes and tumor suppressors 11.34% (11/97), extracellular communication proteins 9.28% (9/97), cell adhesion molecular genes 8.25% (8/97) and the other genes accounted to 32.99% (32/97). JAK1 was up-regulated to 3.78 times, JAK2 to 15.43, STAT1 and STAT2 were up-regulated to 11.98 and 8.11 times respectively, and these genes are components of JAK-STAT pathway. The number of different genes began to decrease on the fifth day. There were still 9 genes that had expression difference more than 3 times on the twenty-first day. It is concluded that when concentration of IFN-alpha was 200 U/ml, the forth day should be considered as the best time to examine change of gene expression in K562 cells treated by IFN-alpha. IFN-alpha realizes its biological functions through the JAK-STAT pathways and it may be one of the mechanisms for curing CML with IFN-alpha.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Regulación Neoplásica de la Expresión Génica / Interferón-alfa / Células K562 / Análisis de Secuencia por Matrices de Oligonucleótidos / Perfilación de la Expresión Génica / Factor de Transcripción STAT1 / Factor de Transcripción STAT2 / Janus Quinasa 1 / Genética Límite: Humanos Idioma: Chino Revista: Journal of Experimental Hematology Año: 2005 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Regulación Neoplásica de la Expresión Génica / Interferón-alfa / Células K562 / Análisis de Secuencia por Matrices de Oligonucleótidos / Perfilación de la Expresión Génica / Factor de Transcripción STAT1 / Factor de Transcripción STAT2 / Janus Quinasa 1 / Genética Límite: Humanos Idioma: Chino Revista: Journal of Experimental Hematology Año: 2005 Tipo del documento: Artículo