Effect of p21-targeted shRNA on curcumin-induced apoptosis of human hepatoma Huh7 cells / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1102-1106, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-344050
ABSTRACT
In the present study, shRNA plasmid of pSi-p21 targeting p21 mRNA was constructed and the effect of p21 shRNA on curcumin-induced apoptosis of human hepatoma Huh7 cells was investigated. The effect of curcumin on the expression of p21 mRNA and protein and the silence efficiency of pSi-p21 were detected with RT-PCR and Western blotting. The effect of pSi-p21 on curcumin-induced apoptosis of Huh7 cells was evaluated with DAPI staining. The results showed that curcumin significantly upregulated p21 mRNA and protein expression, which was knocked down by pSi-p21 of Huh7 cells. DAPI staining results showed that pSi-p21 significantly decreased curcumin-induced apoptosis of Huh7 cells. The data suggested that curcumin induced apoptosis of Huh7 cells via upregulation of p21 expression.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Plásmidos
/
ARN Mensajero
/
Transfección
/
Regulación Neoplásica de la Expresión Génica
/
Regulación hacia Arriba
/
Apoptosis
/
Carcinoma Hepatocelular
/
Curcumina
Límite:
Humanos
Idioma:
Chino
Revista:
Acta Pharmaceutica Sinica
Año:
2009
Tipo del documento:
Artículo
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