Astragalus polysaccharides and astragalosides regulate cytokine secretion in LX-2 cell line / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 543-548, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-344401
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the anti-fibrosis mechanism of radix astragali through the observation on regulating cytokine secretion by astragalosides and astragalus polysaccharides in hepatic stellate cell line LX-2.</p><p><b>METHODS</b>Astragalus polysaccharides and astragalosides at concentration of 25 microg/ml and 300 microg/ml were added to LX-2 cell culture.After 24 h culture total RNA contents were extracted and TGF-beta1, HGF, MP2 and MMP9 mRNA were measured by real time fluorescence quantification PCR technique. The cell growth curves were detected by Real Time Cell Electronics Sensing.</p><p><b>RESULTS</b>300 microg/ml of astragalus polysaccharides suppressed LX-2 cell proliferation (P<0.05, in 94.7 h), while astragalosides induced an significant cell proliferation (P<0.05) both at lower and higher concentration. 25 microg/ml astragalus polysaccharides and astragalosides induced TGF-beta1 expression. For other cytokines, astragalus polysaccharides induced a 104.9 fold higher expression of MMP2, while astragalosides induced a 550.65 fold higher expression of IL-10. Astragalus polysaccharides at 300 microg/ml concentration exhibited an inhibition effect on TGF-beta1, HGF, MMP9 and IL-10 mRNA expression, while up-regulated MMP2 mRNA expression. Astragalosides at 300 mug/ml concentration inhibited TGF-beta1 mRNA expression, while up-regulated MMP2, MMP9 and IL-10 mRNA expression.</p><p><b>CONCLUSION</b>The anti-fibrosis function of astragalus polysaccharides might be associated with up-regulation of MMP2 expression, while that of astragalosides with up-regulation of MMP2, MMP9 and IL-10 expression.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Polisacáridos
/
Saponinas
/
Triterpenos
/
ARN Mensajero
/
Regulación hacia Arriba
/
Línea Celular
/
Química
/
Citocinas
/
Interleucina-10
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2007
Tipo del documento:
Artículo
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