Effect of HanDanGanLe on the cytokines in fibrotic rats / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 285-287, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-344420
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of connective tissue growth factor (CTGF) mRNA and transforming growth factor beta 1 (TGFbeta1) mRNA in immunity-induced liver fibrosis rats and the effect of HanDanGanLe on them.</p><p><b>METHODS</b>Male wistar rats were given intraperitoneal injection of porcine serum twice a week. At the beginning, the rats in HanDanGanLe-treatment group were feed with HanDanGanLe for precaution. The rats were killed after twelve weeks, then CTGF mRNA and TGFbeta1 mRNA were detected in liver samples with in situ hybridization, and the formation of liver fibrosis was observed with HE stain. The semi-quantitative</p><p><b>RESULTS</b>of the two genes expression were analysed along with the stages of hepatic fibrosis.</p><p><b>RESULTS</b>Typical liver fibrosis developed in the model group rats, and the positive stain of CTGF mRNA and TGFbeta1 mRNA increased, which was distributed in the areas where fibrosis occurred. There was obvious correlation between the expression strength of CTGF mRNA and TGFbeta1 mRNA (r = 0.799, P < 0.05). In the rats receiving HanDanGanLe, CTGF mRNA expression index decreased markedly (12.5+/-2.3 vs 28.8+/-1.4, t = 5.208, P < 0.01), so did TGFbeta1 mRNA expression index (25.4+/-3.2 vs 37.3+/-5.4, t = 5.655, P < 0.01). There was also significant correlation between the scores of CTGF mRNA expression and the stages of hepatic fibrosis (rs = 0.822, 0.808 in the model group and HanDanGanLe-treatment group, P < 0.05).</p><p><b>CONCLUSIONS</b>The expression of CTGF mRNA and TGFbeta1 mRNA is correlated closely with hepatic fibrosis degree. HanDanGanLe can effectively prevent the expression of CTGF and TGFbeta1. One of the mechanisms of the intervention may be its blocking the intracellular signalling pathways involved in liver fibrogenesis.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
ARN Mensajero
/
Medicamentos Herbarios Chinos
/
Transducción de Señal
/
Factor de Crecimiento Transformador beta
/
Ratas Wistar
/
Proteínas Inmediatas-Precoces
/
Péptidos y Proteínas de Señalización Intercelular
/
Quimioterapia
/
Factor de Crecimiento Transformador beta1
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Hepatology
Año:
2003
Tipo del documento:
Artículo
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