Synthesis and optimize reaction conditions of chlorogenic acid artificial antigen / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1906-1909, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-344512
ABSTRACT
<p><b>OBJECTIVE</b>To synthesize artificial antigen of the chlorogenic acid (CGA), a constituent in Traditional Chinese Medicine, then optimize the reaction conditions, so that to supply the basis in material and technique for study on Chinese medicine injection of adverse reactions in-depth.</p><p><b>METHOD</b>The complete antigen of chlorogenic acid (CGA-BSA) was synthesized by CGA coupled to carrier protein bovine serum albumi (BSA) using carbodiimide method. We used the orthogonal design test to optimize reaction conditions and used the MALDI-TOF-MS and UV motheds to determine the coupling rate of the CGA-BSA.</p><p><b>RESULT</b>The coupling rate were 20 and 18.8, counting by MALDI-TOF-MS and UV, respectively; while the best reaction conditions were NHS CGA, EDC CGA, CGA BSA were 4 1, 1 1, 200 1, respectively, with the pH 9.</p><p><b>CONCLUSION</b>The complete antigen is preparated successfully. Which supplied the basis to advanced study of correlation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Espectrofotometría Ultravioleta
/
Química
/
Ácido Clorogénico
/
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
/
Antígenos de Plantas
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2009
Tipo del documento:
Artículo
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