Identification of differentially expressed genes in different anomalies of Glycyrrhiza uralensis by cDNA-AFLP method / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To understand the molecular mechanism in genetic variation within species, and identify the differentially expressed genes among 8 anomalies of Glycyrrhiza uralensis under the same genetic background and cultivated environment.</p><p><b>METHOD</b>The differentially expressed genes in roots of different anomalies of G. uralensis in vigorous growing stage were identified by cDNA-AFLP and confirmed by reverse-Northern hybridization. Their functions were inferred through bioinformatics method.</p><p><b>RESULTS</b>Fourteen differentially expressed genes were identified, among which, the function of 6 were unknown, and the rest were involved in antibacterium, regulating metabolism, enhancing the heat resistance ability, increasing the nitrogen fixation ability, and other bioprocesses.</p><p><b>CONCLUSION</b>For the first time, the differentially expressed genes were cloned from the different anomalies of G. uralensis. This provided the basis for the screening of fine anomalies and varieties, and the research of functional genomics.</p>