Influence of serum containing Liuwei Dihuang decoction (see text) on proliferation of osteoblasts under stretch-stress environment / 中国骨伤
China Journal of Orthopaedics and Traumatology
;
(12): 142-146, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-344776
ABSTRACT
<p><b>OBJECTIVE</b>To study influence of serum containing Liuwei Dihuang decoction (see text) on proliferation and differentiation of osteoblast form neonatal SD rats cultured in vitro at different times and different stretch stress.</p><p><b>METHODS</b>After osteoblast cultured for 24 hours in the serum containing Liuwei Dihuang decoction (see text) and serum in control group, the 0.5 Hz frequency, 6% and 12% stretch-stress were added. The MTT1 and the activity of ALP were measured at the 12th and 24th hours, and the data were analyzed.</p><p><b>RESULTS</b>1. In the environment of stretch stress to the frequency of 0.5 Hz, and stretched for 24 hours, the osteoblast was stimulated under elongation rate of 6% and 12%; the proliferation and differentiation of osteoblast was more active under elongation rate of 12% than that of 6%. 2. There were no stimulating effects on osteoblast proliferation and differentiation of serum containing Liuwei Dihuiang decoction (see text) acted on osteoblast cells of SD rats cultured in vitro for a shot time.</p><p><b>CONCLUSION</b>Stretch stress environment can enhance osteoblast proliferation and differentiation cultured in vitro.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Osteoblastos
/
Farmacología
/
Fisiología
/
Estrés Mecánico
/
Medicamentos Herbarios Chinos
/
Diferenciación Celular
/
Ratas Sprague-Dawley
/
Suero
/
Proliferación Celular
/
Fosfatasa Alcalina
Límite:
Animales
Idioma:
Chino
Revista:
China Journal of Orthopaedics and Traumatology
Año:
2013
Tipo del documento:
Artículo
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