High throughput screening of active and stereoselective carbonyl reductases / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 220-230, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-345512
ABSTRACT
In this study, a fast carbonyl reductases colorimetric screening method for discovering stereoselective carbonyl reductases was established by combining the reverse alcohol oxidation with the azoreductase-catalyzed reduction of azo dye. When azo dye (Orange I , 4-(4-hydroxy-1-naphthylazo) benzenesulfonic acid) and azoreductase (AzoB) were added into the reaction system of alcohol oxidation catalyzed by carbonyl reductase, the produced NAD(P)H served as electron donor for the azoreductase to reduce the azo dye, resulting the color fade. Hence, the carbonyl reductases can be screened by the obvious color change. When chiral alcohol was used as the substrate, the activity and stereoselectivity of carbonyl reductases can be screened at the same time.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oxidación-Reducción
/
Estereoisomerismo
/
Compuestos Azo
/
Química
/
Oxidorreductasas de Alcohol
/
Alcoholes
/
Colorantes
/
Ensayos Analíticos de Alto Rendimiento
/
NADH NADPH Oxidorreductasas
/
NADP
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2015
Tipo del documento:
Artículo
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