Construction and characterization of an epitope-mutated Asia 1 type foot-and-mouth disease virus / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 96-104, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-345523
ABSTRACT
To generate an epitope-mutated foot-and-mouth disease virus (FMDV) as a marker vaccine, the infectious clone pAsia 1-FMDV containing the complete genomic cDNA of Asia 1 type FMDV was used as backbone, the residues at positions 27 and 31 in the 3D gene were mutated (H27Y and N31R). The resulting plasmid pAsia 1-FMDV-3DM encoding a mutated epitope was transfected into BHK-21 cells and the recombinant virus rAsia 1-3DM was rescued. The recombinant virus showed similar biological characteristics comparable with the parental virus. In serological neutralization test the antisera against recombine virus have a good reactivity with parental virus. The antisera against the mutant virus were shown to be reactive with the mutated epitope but not the wild-type one. The results indicated that the two virus strains could be distinguished by western blotting using synthetic peptides. This epitope-mutated FMDV strain will be evaluated as a potential marker vaccine against FMDV infections.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Pruebas de Neutralización
/
Transfección
/
Línea Celular
/
ADN Complementario
/
Virus de la Fiebre Aftosa
/
Genética
/
Sueros Inmunes
/
Epítopos
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2015
Tipo del documento:
Artículo
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