Gene expression of AAV-ITR ssDNA mini vector in skeletal muscle of mice / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1720-1732, 2014.
Article
en Zh
| WPRIM
| ID: wpr-345551
Biblioteca responsable:
WPRO
ABSTRACT
AAV-ITR single strand DNA mini vector (AAV-ITR ssDNA mini vector) is a novel gene expression vector based on AAV-ITR. We have shown efficient gene expression of AAV-ITR ssDNA mini vector in HEK 293T. Here, we studied the efficacy of gene expression of AAV-ITR ssDNA mini vector in vivo. We injected the skeletal muscle of ICR mice separately with equal molars of AAV-ITR ssDNA mini vector, ITR mutated AAV-ITR single strand DNA mini vector (AAV-ITRmm ssDNA mutant vector), AAV-ITR dsDNA and pUC57-minivector-GFP, combined with TurboFect. Florescence microscope analysis of skeletal muscle section shows that AAV-ITR ssDNA mini vector had higher expression efficiency and longer expression period. We extracted DNA from the muscle three months after injection and quantified three vectors by Real-time PCR. RT-PCR analysis shows that there were highest copy numbers of AAV-ITR ssDNA mini vector existing in muscle. Stable existing of AAV- TR ssDNA mini vector in muscle could be the molecular basis of long term gene expression of the vector. The results suggest that AAV-ITR ssDNA mini vector might be a promising vector for gene therapy.
Texto completo:
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Índice:
WPRIM
Asunto principal:
ADN de Cadena Simple
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Terapia Genética
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Expresión Génica
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Dependovirus
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Músculo Esquelético
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Reacción en Cadena en Tiempo Real de la Polimerasa
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Vectores Genéticos
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Genética
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Metabolismo
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Ratones Endogámicos ICR
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2014
Tipo del documento:
Article