Preparation and vitality detection of protoplast in Salvia miltiorrhiza Bunge / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1612-1621, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-345562
ABSTRACT
We prepared protoplasts from Salvia miltiorrhiza Bunge suspension culture cells. Then, the protoplasts' vitality and functions were tested by fluorescein diacetate staining method and Fluo-3/AM flourescent probe. The optimal condition of protoplast isolation was Cellulase R-10 1.5%, Pectinase Y-23 0.3%, Macerozyme R-10 0.5%, 40 r/min 12 h, 600 r/min 5 min, and the protoplasts yield was 1.1x10(6) cells/g FW, the vitality was more than 95% by using fluorescein diacetate staining method. It has been confirmed that calcium fluorescent probe Fluo-3/AM can be successfully loaded into protoplasts.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Protoplastos
/
Xantenos
/
Celulasa
/
Química
/
Técnicas de Cultivo de Célula
/
Salvia miltiorrhiza
/
Colorantes Fluorescentes
/
Compuestos de Anilina
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2014
Tipo del documento:
Artículo
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