Evaluation of the iNtRON VRE vanA/vanB Real-Time PCR Assay for Detection of Vancomycin-Resistant Enterococci
Annals of Laboratory Medicine
;
: 76-81, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-34572
ABSTRACT
BACKGROUND:
Recently, the iNtRON VRE vanA/vanB real-time PCR (iNtRON; iNtRON Biotechnology, Korea) assay, a multiplex real-time PCR method, was introduced. In this prospective study, we compared the iNtRON assay with the Seeplex VRE ACE detection kit (Seeplex; Seegene, Korea), a conventional multiplex PCR assay.METHODS:
A chromogenic agar-based culture, in which pre-selected vancomycin-resistant enterococci (VRE) was grown and subsequently plated on blood agar with vancomycin disks, was regarded as the reference method. A total of 304 consecutive rectal swab specimens were tested for VRE by culture and by iNtRON and Seeplex PCR assays. For the PCR assays, specimens were enriched for 16-24 hr before PCR.RESULTS:
VRE were isolated from 44 (14.5%) specimens by chromogenic agar-based culture. The clinical sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the iNtRON assay were 100% (95% confidence interval 89.8%-100%), 99.2% (96.9%-99.9%), 95.6% (83.6%-99.2%), and 100% (98.2%-100%), respectively, while those of the Seeplex assay were 97.7% (86.2%-99.9%), 99.6% (97.5%-99.9%), 97.7% (86.2%-99.9%), and 99.6% (97.5%-99.9%), respectively. The iNtRON assay had a detection limit of 3,159 copies/microL and 13,702 copies/microL for the vanA and vanB genes, respectively. No cross-reactivity was observed in 11 non-VRE bacterial culture isolates.CONCLUSIONS:
The overall performance of the iNtRON assay was comparable to that of a chromogenic agar-based culture method for prompt identification of VRE-colonized patients in hospitals. This assay could be an alternative or supportive method for the effective control of nosocomial VRE infection.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Juego de Reactivos para Diagnóstico
/
Proteínas Bacterianas
/
ADN Bacteriano
/
Técnicas de Tipificación Bacteriana
/
Infecciones por Bacterias Grampositivas
/
Ligasas de Carbono-Oxígeno
/
Resistencia a la Vancomicina
/
Reacción en Cadena en Tiempo Real de la Polimerasa
/
Enterococos Resistentes a la Vancomicina
Tipo de estudio:
Estudio diagnóstico
/
Estudio observacional
Límite:
Humanos
Idioma:
Inglés
Revista:
Annals of Laboratory Medicine
Año:
2015
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS