Evaluation of VITEK Mass Spectrometry (MS), a Matrix-Assisted Laser Desorption Ionization Time-of-Flight MS System for Identification of Anaerobic Bacteria
Annals of Laboratory Medicine
;
: 69-75, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-34573
ABSTRACT
BACKGROUND:
By conventional methods, the identification of anaerobic bacteria is more time consuming and requires more expertise than the identification of aerobic bacteria. Although the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems are relatively less studied, they have been reported to be a promising method for the identification of anaerobes. We evaluated the performance of the VITEK MS in vitro diagnostic (IVD; 1.1 database; bioMerieux, France) in the identification of anaerobes.METHODS:
We used 274 anaerobic bacteria isolated from various clinical specimens. The results for the identification of the bacteria by VITEK MS were compared to those obtained by phenotypic methods and 16S rRNA gene sequencing.RESULTS:
Among the 249 isolates included in the IVD database, the VITEK MS correctly identified 209 (83.9%) isolates to the species level and an additional 18 (7.2%) at the genus level. In particular, the VITEK MS correctly identified clinically relevant and frequently isolated anaerobic bacteria to the species level. The remaining 22 isolates (8.8%) were either not identified or misidentified. The VITEK MS could not identify the 25 isolates absent from the IVD database to the species level.CONCLUSIONS:
The VITEK MS showed reliable identifications for clinically relevant anaerobic bacteria.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Bacterias Anaerobias
/
Líquidos Corporales
/
ARN Ribosómico 16S
/
Técnicas de Tipificación Bacteriana
/
Análisis de Secuencia de ADN
/
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
/
Bases de Datos Genéticas
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Inglés
Revista:
Annals of Laboratory Medicine
Año:
2015
Tipo del documento:
Artículo
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