Optimization of attachment conditions for rabbit mesenchymal stem cells in cytodex 3 microcarrier culture systems / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 884-888, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-346049
ABSTRACT
Mesenchymal stem cells are of great value in tissue engineering and genetic engineering. To study the in vitro dynamic expansion of mesenchymal stem cells by microcarrier technology, we began the research with the investigation of attachment of rabbit mesenchymal stem cells on cytodex 3 microcarriers after inoculation. The result showed a poor attachment efficiency of 16.7% +/- 1.1% under general conditions, so the attachment efficiency must be increased through the optimization of inoculation conditions. Intermittent stirring, inoculation in 50% of the final culture volume and reduction of the fetal bovine serum concentration at inoculation all led to notable increases in attachment efficiency. Ratio of rMSCs attached in aMEM cultures was 39.8% higher than that of DMEM's on the average. When all these optimal conditions were adopted, the attachment efficiency (65.5%) was significantly higher than that (26.6%) under general conditions; accordingly the final growth extents of rMSCs were 4. 50 and 2. 01, respectively. Therefore, the optimization of attachment conditions promoted the expansion of rMSCs on microcarriers.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Células de la Médula Ósea
/
Dextranos
/
Técnicas de Cultivo de Célula
/
Biología Celular
/
Proliferación Celular
/
Células Madre Mesenquimatosas
/
Métodos
/
Microesferas
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Biomedical Engineering
Año:
2007
Tipo del documento:
Artículo
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