Transfection of the nm23-H1 gene into BcaCD885 cell line inhibits the potential of invasion, adhesion and mobility / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 16-19, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-347316
ABSTRACT
<p><b>OBJECTIVE</b>To transfect nm23-H1 into the BcaCD885 cell lines in order to get safe high-efficiency and low-toxicity, and to find out whether nm23-H1 could affect the invasion and metastases ability of BcaCD885 cell lines.</p><p><b>METHODS</b>Lipofect was used to transfect nm23-H1 into BcaCD885 cell lines; immunohistochemistry was used to detect the difference expression of nm23-H1 between transfected and non-transfected cell lines; then transwell-room and wash way were used to detect the difference of invasion and metastases ability between transfected and non-transfected cell lines.</p><p><b>RESULTS</b>PCMV-NEO-BAM system gave the stability expression of nm23-H1; there was significant different NDPKA expression between transfected and non-transfected BcaCD885 cell lines; the invasion and metastases ability of transfected BcaCD885 cell lines decreased obviously.</p><p><b>CONCLUSION</b>nm23-H1 can inhibit the metastases of BcaCD885 cell lines significantly.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Fisiología
/
Factores de Transcripción
/
Neoplasias de la Boca
/
Células Tumorales Cultivadas
/
Transfección
/
Adhesión Celular
/
Movimiento Celular
/
Nucleósido-Difosfato Quinasa
/
Proteínas de Unión al GTP Monoméricas
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Stomatology
Año:
2003
Tipo del documento:
Artículo
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