Expression of microRNA in neonatal rats with hypoxic-ischemic brain damage / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 373-376, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-347595
ABSTRACT
<p><b>OBJECTIVE</b>To study the changes of microRNA expression in cortex tissues in neonatal rats with hypoxic-ischemic brain damage (HIBD)and the possible roles of microRNA in the pathogenesis of HIBD. METHODS Rat HIBD model was prepared. The cortex tissues were obtained 14 days after the HIBD event. The microRNA expression profiles were measured using microRNA microarray. Expression of 9 microRNAs (miR-126,-26a,-674-5p,-21,-25,-290, miR-124,-125b-5p and microRNA-9a) was determined by quantitative real-time PCR.</p><p><b>RESULTS</b>he results of microRNA expression profiles indicated that 27 pieces of microRNA were up-regulated more than 2 folds and 60 pieces were down-regulated more than 2 folds compared with the normal control group. The results of the 9 microRNAs detected by quantitative real-time PCR were consistent with those detected by microRNA microarray.</p><p><b>CONCLUSIONS</b>HIBD rats have significant changes in microRNA expression, suggesting that microRNA expression may play important roles in the pathogenesis of HIBD.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Ciclo Celular
/
Reacción en Cadena de la Polimerasa
/
Ratas Sprague-Dawley
/
Apoptosis
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Hipoxia-Isquemia Encefálica
/
MicroARNs
/
Genética
/
Animales Recién Nacidos
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Contemporary Pediatrics
Año:
2010
Tipo del documento:
Artículo
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