Influence of Wnt-1 recombinant adenovirus on differentiation of human epidermal stem cells / 中华烧伤杂志
Chinese Journal of Burns
;
(6): 187-190, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-347618
ABSTRACT
<p><b>OBJECTIVE</b>To observe the influence of Wnt-1 recombinant adenovirus on differentiation tendency of human epidermal stem cells.</p><p><b>METHODS</b>Wnt-1 recombinant adenovirus was transduced into hESCs (E group), while normal hESCs were used as control (C) group. The diameter, proliferation,and labeling molecular expression of hESC were determined. The content of MMP-2 and MMP-7 in supernate were also assayed.</p><p><b>RESULTS</b>There was no obvious difference in diameter of hESC between two groups. The density of hESC in E group was (1.45 +/- 0.09) x 10(5)/mL, which was obviously higher than that in C group [(1.18 +/- 0.10) x 10(5)/mL, P < 0.05]. There were no obvious differences in expression of markers between two groups,including keratin 5 (KS), K6, K7, KS, K14, CD44, carcinoembryonic-like antigen (CEAA), ER, PR (P > 0.05) ,while the expression of K 10 was different among groups [(60 +/- 3)% in E group, 0 in C group], also K18 [(34.3 +/- 2.1)% in E group vs. (13.8 +/- 1.7)% in C group, P < 0.05], and K19 [(17.1 +/- 1.8)% in E group vs. (24.4 +/- 1.5)% in C group, P < 0.05].The contents of MMP-2 and MMP-7 in E group were higher than those in C group (P < 0.01).</p><p><b>CONCLUSION</b>Wnt-1 recombinant adenovirus can induce the differentiation of hESCs to glandular epithelium-like cells.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Células Madre
/
Virología
/
Diferenciación Celular
/
Línea Celular
/
Adenoviridae
/
Metaloproteinasa 2 de la Matriz
/
Metaloproteinasa 7 de la Matriz
/
Biología Celular
/
Células Epiteliales
/
Proteína Wnt1
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Burns
Año:
2008
Tipo del documento:
Artículo
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