Study on Acinetobacter baumannii plasmid with 3 types of beta-lactamase genes in a burn ward / 中华烧伤杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the transferrable character of Acinetobacter baumannii (AB) plasmids with 3 types of beta-lactamase gene.</p><p><b>METHODS</b>The plasmid of multi-drug resistant AB (donor) isolated from burn wound were transferred to E. coil ATCC25922 (receptor) through conjugation, and drug sensitivity was also observed. Drug-resistant gene and stability of filial generation and zygote were analyzed by PCR.</p><p><b>RESULTS</b>The drug-resistance of donor plasmids to Sulfamethoxazole, Ampicillin, Cefalotin, Cefpodoxime, Cefuroxime, Imipenem/Cilastatin and Ampicillin/Sulbactam, and three types of beta-lactamase gene were transferred to the receptor, and were also stably transmitted for passages. The minimum inhibitor concentration of receptor to Sulfamethoxazole was > 2 mg/L after conjugation with donor, and inhibitory character could be transferred to next generation.</p><p><b>CONCLUSION</b>bla(TEM-1), bla(PER-1) and bla(OXA-23) genes carried in the plasmid of AB can be transferred through conjugation and stably transmitted for passages, and it is one of the molecular mechanisms for AB with multi-drug resistance after burn infections.</p>