Effect of phytohemagglutinin on proliferation and cytotoxicity of cytokine-induced killer cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 118-120, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-347814
ABSTRACT
The purpose was to investigate the effect of phytohemagglutinin (PHA) on proliferation and cytotoxicity of cytokine-induced killer (CIK). Peripheral blood mononuclear cells (PBMNCs) from healthy donors were divided into two groups. Cells were resuspended and maintained in complete medium containing of 10% autologous plasma. CIK cells were cultured by traditional method in group one. The other group cells were added PHA to stimulate PBMNCs for 24 hours, then cultured like incubating CIK cells. Their cytotoxicity to different target cells was evaluated by (51)Cr release assay. The results showed that the proliferation multiples of CIK and PHA-CIK cells were both high, however, the latter was much higher than CIK with significance (P < 0.05). Cells in each group cells showed high cytotoxicity. At the same high effector/target ratio PHA-CIK cells cytotoxicity was stronger than CIK cells when targets were K562 cells or acute leukemia cells (P < 0.05). In conclusion, PHA-CIK cells exhibit stronger proliferation and cytotoxicity than CIK cells, and the result provides an experimental basis for biotherapy.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Fitohemaglutininas
/
Pruebas Inmunológicas de Citotoxicidad
/
Leucocitos Mononucleares
/
Células Asesinas Activadas por Linfocinas
/
Células Cultivadas
/
Técnicas de Cocultivo
/
Células K562
/
Biología Celular
/
Citotoxicidad Inmunológica
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2005
Tipo del documento:
Artículo
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