Effects of HGF on GVHD and Th1/Th2-related cytokines in ALL mice after allo-BMT / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 35-38, 2005.
Article
en Zh
| WPRIM
| ID: wpr-347831
Biblioteca responsable:
WPRO
ABSTRACT
To observe the effects of hepatocyte growth factor (HGF) on graft-versus-host disease (GVHD) and Th1/Th2 related cytokines in mice with acute lymphoblastic leukemia (ALL) after allogenic bone marrow transplantation (allo-BMT), BALB/c mice were conditioned by total body irradiation with 11 Gy and then were transplanted with allogeneic bone marrow after establishing ALL model. BALB/c mice were divided into groups A and B. The mice of group A were injected subcutaneously with HGF from day 0 to 7 after allo-BMT, and the mice of group B were injected subcutaneously with PBS from day 0 to 7 after allo-BMT. The symptoms of GVHD and the GVHD pathological changes of liver and small intestine and skin were observed. The serum levels of both IFN-gamma and IL-4 were determined by ELISA. The results showed that the score of GVHD in group A was lower than that in group B (P < 0.05). The levels of IFN-gamma in both groups A and B were all higher than that in normal group (P < 0.05 and P < 0.001, respectively), However, the level of IFN-gamma in group A was lower than that in group B (P < 0.01). The levels of IL-4 in both group A and B were all lower than that in normal group (P < 0.05), but the level of IL-4 in group A was higher than that in group B (P < 0.05). It is concluded that HGF can alleviates the severity of GVHD, because of its balancing the Th1/Th2-related cytokines after allo-BMT.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Farmacología
/
Cirugía General
/
Trasplante Homólogo
/
Sangre
/
Ensayo de Inmunoadsorción Enzimática
/
Citocinas
/
Trasplante de Médula Ósea
/
Interleucina-4
/
Interferón gamma
/
Factor de Crecimiento de Hepatocito
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2005
Tipo del documento:
Article