Establishment and evaluation on the method for platelet intracellular trehalose determination / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 837-840, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-347850
ABSTRACT
This study was aimed to establish an applicable, convenient and rapid method for platelet intracellular trehalose determination, so that the technique of trehalose loading could be optimized and used in research on freeze-drying platelets. Protein from loaded-trehalose platelets was deposited by using trichloroacetic acid, trechalose concentration in platelets was determinated by sulfuric-anthrone reaction and was analyzed by high performance liquid chromatography (HPLC). The results showed that when platelets were loaded with 1.7% of trehalose in loading solution, intracellular concentration determinated by sulfuric-anthrone reaction was 0.22% and derterminated by HPLC was 0.2%. The recovery rate of trehalose determinated by sulfuric-anthrone reaction was 100.7% and stability and repeatability of results were good. In conclusion, the method is convenient, rapid, accurate and highly sensitive for determination of platelet intracellar trehalose.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Ácidos Sulfúricos
/
Trehalosa
/
Plaquetas
/
Química
/
Reproducibilidad de los Resultados
/
Cromatografía Líquida de Alta Presión
/
Métodos
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2004
Tipo del documento:
Artículo
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