Inhibition effect of bone marrow mesenchymal stem cells on T-lymphocyte proliferation through up-regulation of CD8+CD28- T cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 666-669, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-347889
ABSTRACT
The aim of this study was to explore effect of CD8+CD28- T-lymphocyte in the inhibition of mesenchymal stem cells (MSC) on T-lymphocyte proliferation. T cells were harvested by using nylon column and CD8+ T cells were sorted by magnetic beads; the T-lymphocyte proliferation in the presence of PHA was evaluated by MTT; the proportion of CD8+CD28- T cells was assayed by fluorescence-activated cell sorter (FACS). The results showed that MSC inhibited T-lymphocyte proliferation and the inhibitory effect depended on the amount of MSC; the data of FACS indicated that in the CD8+ T cells co-cultured with MSC, CD8+CD28- T cells were up-regulated significantly, compared with the non-treated CD8+ T cells. In conclusion, MSC perform their immunosuppressive function by up-regulation of CD8+CD28- T cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Células de la Médula Ósea
/
Activación de Linfocitos
/
Regulación hacia Arriba
/
Subgrupos de Linfocitos T
/
Antígenos CD8
/
Antígenos CD28
/
Alergia e Inmunología
/
Células Madre Mesenquimatosas
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2004
Tipo del documento:
Artículo
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