Expression of basic fibroblast growth factor and nuclear factor-kappaB and the effect of budesonide on their expression in rats with asthma / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 393-396, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-347906
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of basic fibroblast growth factor (b-FGF) and nuclear factor-kappaB (NF-kappaB) in the airway and the effect of budesonide on their expression in rats with asthma.</p><p><b>METHODS</b>Forty-five Sprague-Dawley male rats were randomly divided into three group placebo control, untreated asthma, and budesonide-treated asthma. Asthma was induced by intraperitoneal injection of 10% ovalbumin (OVA) on days 1 and 8 and then challenged by inhalation of 1% OVA aerosol. The budesonide-treated asthma group received an inhalation of budesonide (1 mg) 30 minutes after OVA challenge. The pathological changes of the airway were assessed, and the expression of b-FGF and NF-kappaB in the airway was assayed by hematoxylin and eosin staining and immunohistochemistry.</p><p><b>RESULTS</b>Budesonide treatment alleviated airway injuries. Compared with the control group, b-FGF and NF-kappaB expression in the airway in the untreated asthma group increased significantly (P< 0.05). The budesonide-treated asthma group demonstrated significantly decreased b-FGF (111.61+/- 5.52 vs 126.21+/- 6.46; P< 0.05) and NF-kappaB expression (110.65+/- 8.71 vs 134.15+/- 9.42; P< 0.05) in the airway as compared with the untreated asthma group. B-FGF expression was positively correlated to NF-kappaB expression in the budesonide-treated group.</p><p><b>CONCLUSIONS</b>b-FGF and NF-kappaB may be associated with airway remodeling in rats with asthma. Budesonide can improve airway remodeling, possibly by decreasing the expression of b-FGF and NF-kappaB.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Asma
/
Bronquios
/
Inmunohistoquímica
/
Factor 2 de Crecimiento de Fibroblastos
/
FN-kappa B
/
Ratas Sprague-Dawley
/
Budesonida
/
Metabolismo
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Contemporary Pediatrics
Año:
2009
Tipo del documento:
Artículo
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