Cloning of human bone morphogenetic protein-2 gene and the construction of its eukaryotic expression vector / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 487-489, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-348012
ABSTRACT
<p><b>OBJECTIVE</b>To clone human bone morphogenetic protein-2 (hBMP2) gene and construct its eukaryotic expression vector pcDNA3.1 -hBMP2.</p><p><b>METHODS</b>Human BMP2 gene was amplified by RT-PCR method from human osteosarcoma cells and constructed into eukaryotic expression vector pcDNA3.1-hBMP2. The gene in the vector pcDNA3.1-hBMP2 was identified by PCR amplification, enzyme digestion and DNA sequencing.</p><p><b>RESULTS</b>The cloned DNA was confirmed to be hBMP-2 gene.</p><p><b>CONCLUSION</b>In this study, hBMP2 gene is successfully cloned and its eukaryotic expression vector pcDNA3.1-hBMP2 is constructed, which provides the foundation of using BMP2 gene therapy to accelerate new bone formation in distraction osteogenesis.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Transfección
/
Terapia Genética
/
Reacción en Cadena de la Polimerasa
/
Análisis de Secuencia de ADN
/
Clonación de Organismos
/
Proteína Morfogenética Ósea 2
/
Vectores Genéticos
Límite:
Humanos
Idioma:
Chino
Revista:
West China Journal of Stomatology
Año:
2007
Tipo del documento:
Artículo
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