Effect of astragalus polysaccharides on the proliferation and ultrastructure of dog bone marrow stem cells induced into osteoblasts in vitro / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 432-436, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-348027
ABSTRACT
<p><b>OBJECTIVE</b>To observe the growth and osteogenic property of cultured dog bone marrow stem cells (BMSCs) by investigating the effects of astragalus polysaccharides (APS) on the proliferation and ultrastructure of BMSCs into osteoblasts in vitro.</p><p><b>METHODS</b>BMSCs osteogenic property was detected by improved Wright-Giemsa, Gomori and alizarin dyeing method. The proliferation and differentiation of the induced BMSCs with APS in different concentration and time were detected by MTT assay and the morphologic change of the induced BMSCs was observed by transmission electron microscope (TEM).</p><p><b>RESULTS</b>BMSCs osteogenic property was detected with Wright-Giemsa deep-bluing, Gomori method blacking and with more mineral nodules alizarin dyeing method carmining. APS with concentration of 0.005 mg/mL can promote the proliferation of the induced BMSCs in short-term culture (1th, 3th day) and 50 mg/mL can decrease the effect through long-term culture (5th day). Observed by TEM (5th day), the number of mitochondria, rough endoplasmic reticulum increased and the extracellular matrix was excreted more in the induced BMSCs by APS with concentration of 0.005 mg/mL. However, not only the number of mitochondria, rough endoplasmic reticulum reduced but also the structure was swollen, degenerative, membrance damaged in the induced BMSCs by APS with concentration of 50 mg/mL.</p><p><b>CONCLUSION</b>APS with lower concentration in short-term culture may promote BMSCs proliferation and differentiation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Osteoblastos
/
Polisacáridos
/
Técnicas In Vitro
/
Células de la Médula Ósea
/
Diferenciación Celular
/
Células Madre Mesenquimatosas
Límite:
Animales
Idioma:
Chino
Revista:
West China Journal of Stomatology
Año:
2007
Tipo del documento:
Artículo
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