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Initial study on discrimination of oral microorganisms with the metabonomics technique / 华西口腔医学杂志
West China Journal of Stomatology ; (6): 342-344, 2007.
Artículo en Chino | WPRIM | ID: wpr-348053
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the feasibility of employing metabonomics method in identification of oral pathogenic bacteria.</p><p><b>METHODS</b>The Streptococcus mutans ATCC25175 and Actinomyces viscosus ATCC15987 were respectively inoculated in same certain culture medium. The growth curves of the inoculated bacteria were drown by turbidimetry. The culture solutions in four different growth phases of the both bacteria were used to test with the 1H-Nuclear magnetic resonance (1H-NMR) spectroscopy respectively. The data of 1H-NMR spectroscopy results were analyzed by principal components analysis (PCA).</p><p><b>RESULTS</b>The PCA showed the obvious clustering phenomena and the points of two group data stayed differentially together by two clusters. Therefore, the NMR-based metabonomics profiles can discriminate the two different kind of bacteria.</p><p><b>CONCLUSION</b>The metabonomics can be expected to be a kind of promising useful method in quick discrimination of oral pathogenic bacteria.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Streptococcus mutans / Espectroscopía de Resonancia Magnética / Medios de Cultivo / Metabolómica Tipo de estudio: Estudio pronóstico Idioma: Chino Revista: West China Journal of Stomatology Año: 2007 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Streptococcus mutans / Espectroscopía de Resonancia Magnética / Medios de Cultivo / Metabolómica Tipo de estudio: Estudio pronóstico Idioma: Chino Revista: West China Journal of Stomatology Año: 2007 Tipo del documento: Artículo