Effect of focal adhesion kinase on biological characteristics of Tca8113 tongue squamous cell carcinoma / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of FAK on biological characteristics of tongue squamous cell carcinoma cell Tca8113.</p><p><b>METHODS</b>Lipofect was used to transfect FAK into Tca8113 cell lines. The difference in expression of FAK between the transfected and non-transfected cell lines was detected by immunofluorescence assay. Then by use of transwell room and wash way, the difference in invasion and adhesion ability between the transfected and non-transfected cell lines was tested. MTT method was adopted in finding the change of proliferation.</p><p><b>RESULTS</b>RT-PCR analysis showed that the FAK gene was downregulated by Tca8113 cells. FAK expression was significantly down regulated by the transfection of FAK ASODN (P<0.05). Antisense FAK ODN significantly inhibited proliferation, adhesion and the invasion of Tca8113 cells.</p><p><b>CONCLUSION</b>FAK plays an important role on biological characteristics of tongue squamous cell carcinoma cell Tca8113.</p>