Your browser doesn't support javascript.
loading
Dendritic cell vaccine modified by murine mAFP gene enhances immunoprotective effect on liver carcinogenesis and tumor development in mice / 中华肿瘤杂志
Chinese Journal of Oncology ; (12): 250-254, 2008.
Artículo en Chino | WPRIM | ID: wpr-348120
ABSTRACT
<p><b>OBJECTIVE</b>To construct a dendritic cell vaccine transduced by murine alpha-fetoprotein (mAFP) gene, and evaluate its immunoprotective effect on C57BL/6J mice during the induction of hepatocellular carcinoma by diethylnitrosamines, carbon tetrachloride and ethanol.</p><p><b>METHODS</b>Dendritic cells (DCs) were induced and augmented by murine IL-4 and GM-CSF, and transfected by recombinant adenovirus engineered with mAFP gene. Major MHC class I and II, B7.1 (CD80), B7.2 (CD86), CD18a, and CD54 molecules on DC were analyzed by FACS. 80 C57BL/6J male mice were randomly divided into 4 groups (20 mice per group) Simple DC inoculated group, pAdBM5-mAFP-DC inoculated group, pAdBM5-mAFP plasmid inoculated group, and PBS control group. They were immunized once with 5 x 10(5) DCs (0.1 ml)/mouse administered s. c. in the left flank or 100 mg pAdBMS-mAFP plasmid/mouse administered i. m. in the left tibialis anterior muscle. Inoculation was conducted once a week for 4 weeks after 3 times consecutive immunization initially. At the same time of immunization, DEN/CCl4/ethanol were given to induce hepatocellular carcinoma. Tumor incidence was assessed after 20 weeks.</p><p><b>RESULTS</b>A transgenic DC vaccine was successfully constructed and the mAFP transgenic DCs expressed high level molecules of major MHC class I and II , B7.1, B7.2, CD18a, and CD54. After the 20-week induction, the incidence of primary hepatocellular carcinoma (PLC) was 70.0% in simple DC inoculated group, 25.0% in pAdBMS-mAFP-DC inoculated group, 65.0% in pAdBM5-mAFP plasmid inoculated group, and 75.0% in PBS control group. There was a significant difference between group B and other groups (P < 0.05).</p><p><b>CONCLUSION</b>mAFP transgenic DC tumor vaccine inoculation may induce strong immunoprotection against liver carcinogenesis and tumor development and reduce PLC incidence induced by DEN/CCl4/ethanol.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Células Dendríticas / Proteínas Recombinantes / Tetracloruro de Carbono / Transfección / Antígenos de Histocompatibilidad Clase I / Alfa-Fetoproteínas / Distribución Aleatoria / Antígenos de Histocompatibilidad Clase II / Células Cultivadas / Adenoviridae Límite: Animales Idioma: Chino Revista: Chinese Journal of Oncology Año: 2008 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Células Dendríticas / Proteínas Recombinantes / Tetracloruro de Carbono / Transfección / Antígenos de Histocompatibilidad Clase I / Alfa-Fetoproteínas / Distribución Aleatoria / Antígenos de Histocompatibilidad Clase II / Células Cultivadas / Adenoviridae Límite: Animales Idioma: Chino Revista: Chinese Journal of Oncology Año: 2008 Tipo del documento: Artículo