Zinc finger nucleases and their application / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 162-165, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-349020
ABSTRACT
Zinc finger nuclease (ZFN), which is a chimeric fusion structure between a Cys2-His2 zinc-finger protein (ZFP) and the cleavage domain of Fok I endonuclease, can be used to introduce targeted double-stranded breaks (DSBs). ZFN-mediated cleavage leads to mutations when double-stranded breaks are repaired by homologous recombination (HR) or nonhomologous end joining (NHEJ). In recent years, ZFNs are widely used in the fields of genetic research. In this review, the methodology and technical advantages of ZFNs were briefly discussed.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Desoxirribonucleasas de Localización Especificada Tipo II
/
Química
/
Dedos de Zinc
/
Genética
/
Metabolismo
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Medical Genetics
Año:
2010
Tipo del documento:
Artículo
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