Generation of factor VIII gene knockout mouse by tetraploid embryo complementation technology / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>Factor VIII( FVIII) gene knockout mouse model was established for further study on the treatment of hemophilia A.</p><p><b>METHODS</b>Exons 16-19 of the mouse FVIII gene were knocked out by ET clone, ES homologous recombination and tetraploid embryo compensation technology. PCR, reverse transcriptase-PCR(RT-PCR) and immunohistochemistry were used to detect the transcription and translation pattern of FVIII. The phenotype of the knockout mice was analyzed by examining the activated partial thromboplastin time (APTT) and FVIII activity (FVIIIC).</p><p><b>RESULTS</b>PCR, RT-PCR and immunohistochemistry confirmed that FVIII was deficient in the FVIII gene knockout mouse. The APTT results showed that FVIII-deficient mouse plasma had a prolonged clotting time compared to normal mouse plasma. The FVIIIC in heterozygous, hemizygous and homozygous mice was 80%, 8% and 10% of that in normal mice, respectively.</p><p><b>CONCLUSION</b>The phenotype of the FVIII gene knockout mouse appears grossly similar to that of human with hemophilia A. Establishment of this model may promote the development of new technologies of treatment to hemophilia A.</p>