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Rapid screening of katG gene mutation in isoniazid-resistant Mycobacterium tuberculosis / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences ; (6): 178-180, 2002.
Artículo en Chino | WPRIM | ID: wpr-349448
ABSTRACT

OBJECTIVE:

To evaluate the relationship between katG gene mutation and isoniazid (INH) resistance and to develop a rapid screening method of point mutation in the katG gene associated with MTB resistance.

METHODS:

Twenty-four clinical isolates of MTB with 8 INH resigtance isolates and 16 INH-sensitive isolates were analyzed by PCR-RFLP, with the H(37)Rv reference strain as the control.

RESULTS:

G-->C point mutations were detected in 7 of 8 isoniazid-resistant strains and no gene mutation was shown in 16 isoniazid-sensitive isolates. The sensitivity and specificity were 87.5 % and 100 % respectively. No katG gene sequence deletion was observed in any specimen.

CONCLUSION:

Our results suggest katG gene mutation is one of the most important mechanisms of INH-resistant TB. PCR-RFLP may be useful in detection of katG gene mutation.
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Tipo de estudio: Estudio diagnóstico / Estudio de tamizaje Idioma: Chino Revista: Journal of Zhejiang University. Medical sciences Año: 2002 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Tipo de estudio: Estudio diagnóstico / Estudio de tamizaje Idioma: Chino Revista: Journal of Zhejiang University. Medical sciences Año: 2002 Tipo del documento: Artículo