Discrimination of alleles in HLA-C*04:01:01G groups / 中国实验血液学杂志

ABSTRACT

The aim of this study was to investigate the relatively frequencies of alleles in the HLA-C*040101G group and to analyze their relations with HLA-A and -B loci. DNA samples previously typed as HLA-C*040101G were sequentially selected. The sequences for exon 2 to 7 of the HLA-C locus were analyzed by polymerase chain reaction sequence-based typing(PCR-SBT). The HLA-A, -B, -DRB1 and -DQB1 loci were genotyped using PCR-SBT method. The results showed that 178 samples (94.2%) and 11 samples (5.8%) were assigned as HLA-C*040101 and HLA-C*0482 respectively among 189 samples previously typed as HLA-C*040101G. 72 haplotypes associated with HLA-C*040101 and C*0482 were found, in which the frequencies of 26 haplotypes were over 0.0050. HLA-C*040101 was strongly related with A*0203, A*0207, A*1101, A*3303, B*1301, B*1501, B*1505, B*1527, B*4001, B*5401 alleles, while HLA-C*0482 was related with B*4001. It is concluded that HLA-C*040101 and HLA-C*0482 alleles were confirmed in the HLA-C*040101G group, which should be discriminated by the routine HLA genotyping.