Identification of a novel HLA allele, HLA-DRB1*03:80, by sequencing-based typing / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 509-512, 2014.
Article
en Zh
| WPRIM
| ID: wpr-349681
Biblioteca responsable:
WPRO
ABSTRACT
This study was aimed to identify a novel HLA-DRB1 allele from a Chinese potential hemopoietic stem cell donor of Northeast China. A rare HLA-DRB1 allele was initially detected by Luminex PCR-SSO typing, then the sample was sequenced by sequence-based typing (SBT) and the alignments of sample's alleles was identified by single allele-specific sequencing strategy. The results revealed the existence of a new allele which differs from the closest matching allele DRB1*03:06 by a single nucleotide substitution at position 239, where C→G in exon 2, resulting in an amino acid exchange from Thr to Arg at codon 51. It is concluded that a novel allele has been confirmed and its name DRB1*03:80 is officially assigned by the WHO Nomenclature Committee in February 2012.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Análisis de Secuencia de ADN
/
Pueblo Asiatico
/
Alelos
/
Cadenas HLA-DRB1
/
Genética
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
/
Male
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2014
Tipo del documento:
Article