Cloning and characterization of a novel rat gene RSD-7 differentially expressed in testis / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 289-293, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-350107
ABSTRACT
<p><b>OBJECTIVE</b>To isolate and identify the differentially expressed genes in spermatogenesis for the understanding molecular mechanism of spermatogenesis.</p><p><b>METHODS</b>Screening of the cDNA library, Northern blot, expression and purification in E. coli with GST expression system, immunocytochemical staining of testis sections were used.</p><p><b>RESULTS</b>(1) A cDNA fragment designated as RSD-7 was isolated from rat testis cDNA library. It was 1,238 bp in length, coding a protein of 232 amino acids with the GenBank accession number AF315467. The encoding protein of RSD-7 cDNA had a Ubiquitin-like domain. (2) Northern blot indicated that RSD-7 was uniquely expressed in rat testis, and in the testis RSD-7 emerged on the 30th postnatal day and expressed until 120th postnatal day. (3) Expression and purification of RSD-7 protein in E. coli with GST expression system and were used to obtain anti-RSD-7 antibody. (4) Immunolocalization of RSD-7 in rat testis revealed that it is expressed only in Sertoli cells.</p><p><b>CONCLUSIONS</b>Transcription pattern of RSD-7 and localization of RSD-7 protein in testis have been made, which established the base for the functional study of RSD-7.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Represoras
/
Células de Sertoli
/
Espermatogénesis
/
Testículo
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Ubiquitinas
/
Secuencia de Aminoácidos
/
Clonación Molecular
/
Ratas Wistar
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Acta Academiae Medicinae Sinicae
Año:
2003
Tipo del documento:
Artículo
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