Determination of salidroside and p-tyrosol in Hongjingtian for injection(freezing-dry) by SPE-HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To assay salidroside and p-tyrosol in Hongjingtian for injection (freezing-dry).</p><p><b>METHOD</b>Samples were purified by Sep-Pak C18 column and salidroside and p-tyrosol were determined by HPLC with Irregular-H C18 (4.6 mm x 250 mm, 5 microm), and eluted with a mobile phase of methanol-acenitonitrile -0.06% phosphate (10 1080). The flow rate was 1.0 mL x min(-1), the detection wavelength was set at 275 nm and the column temperature was maintained at 30 degrees C.</p><p><b>RESULT</b>The calibration curves were linear in the range of 2.24-22.4 microg for salidroside (0.999 7) and 0.856-8.56 microg for p-tyrosol (0.999 6), the average recovery was 101.3%, 99.8% respectively.</p><p><b>CONCLUSION</b>The method is convenient, rapid, accurate and reliable.</p>