The relationship between endoplasmic reticulum stress and its particular apoptosis way caspase-12 and apoptosis in renal cortex of diabetic rats / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 236-240, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-351189
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expressions of 78-kDa glucose-regulated protein (GRP78) and Caspase-12 and their relationship with apoptosis in renal cortex of diabetic rats.</p><p><b>METHODS</b>Uninephrectomized Wistar rats were used to induce diabetes by intraperitoneal injection of Streptozotocin (STZ 65 mg/kg). After 8 weeks, the expression and distribution of GRP78, Caspase-12, proliferating cell nuclear antigen (PCNA) were examined by immunohistochemistry. Flow cytometry was used to detect the levels of protein of GRP78 and Caspase-12. Apoptosis was evaluated by means of terminal deoxynucleotidyl transferase-mediated d-UDP nick-end labeling (TUNEL) and Flow cytometry. Serum creatinine, blood urea nitrogen and 24-hour urine protein excretion were checked.</p><p><b>RESULTS</b>Compared with those in normal control group, the numbers of apoptosis and the expression of GRP78, Caspase-12 in glomerular and tubular cells were much higher in the diabetic kidneys at 8 weeks. There was no significant difference between group A and group B.</p><p><b>CONCLUSION</b>Activation of endoplasmic reticulum stress may play an important role in the development of diabetic nephropathy.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Ratas Wistar
/
Apoptosis
/
Antígeno Nuclear de Célula en Proliferación
/
Diabetes Mellitus Experimental
/
Nefropatías Diabéticas
/
Caspasa 12
/
Estrés del Retículo Endoplásmico
/
Proteínas de Choque Térmico
/
Corteza Renal
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2011
Tipo del documento:
Artículo
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