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Analysis of porcine preadipocytes differentiation by atomic force microscope / 生物工程学报
Chinese Journal of Biotechnology ; (12): 124-130, 2011.
Artículo en Chino | WPRIM | ID: wpr-351526
ABSTRACT
Abnormal changes during fat formation are closely related to the prevalence of many diseases. In order to understand the formation mechanism of fat, we used atomic force microscopy (AFM) to characterize the morphology and mechanical properties of porcine preadipocytes during the differentiation. Preadipocytes and adipocytes were different morphologically. The surface roughness of adipocytes was less than preadipocytes by detection of the ultrastructure. The mechanical properties of preadipocytes were changed during differentiation with AFM-based force spectroscopy. Preadipocytes were 20% higher than adipocytes in the adhesion force, stiffness and Young's modulus. Therefore, AFM analysis of membrane changes related to adipocytes formation provided quantitative data in the nanometer level for further studying the formation mechanism of the adipocytes.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fisiología / Porcinos / Diferenciación Celular / Células Cultivadas / Adipocitos / Microscopía de Fuerza Atómica / Biología Celular / Adipogénesis Límite: Animales Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2011 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fisiología / Porcinos / Diferenciación Celular / Células Cultivadas / Adipocitos / Microscopía de Fuerza Atómica / Biología Celular / Adipogénesis Límite: Animales Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2011 Tipo del documento: Artículo