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Effects of solvent environment on the structure of hepatitis B surface antigen (HBsAg) / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1674-1682, 2010.
Artículo en Chino | WPRIM | ID: wpr-351549
ABSTRACT
As a virus-like particle, hepatitis B surface antigen (HBsAg) was the primary component of hepatitis B vaccine. HBsAg was maintained by the non-covalent interaction of proteins and lipids. The intact structure of HBsAg particle was vital to its function. However, there was no report about the effects of solvent environment on HBsAg structure. In this paper, we studied the effects of temperature, pH, ionic type and salt concentration on HBsAg structure. The results showed that HBsAg was stable at normal temperature, but began to denature above 60 degrees C. The aggregation of HBsAg at pH 3.0 and 4.0 was nearly irreversible, but partly reversible at pH 5.0. The influence of ionic type on HBsAg was generally in accordance with Hofmeister sequence, except that SO4(2-) caused more aggregation than F-. HBsAg aggregates started to be visible in 0.4 mol/L (NH4)2SO4, and the extent of aggregation increased with the salt concentration. Therefore, caution must be taken when using (NH4)2SO4 in the hydrophobic chromatography purification of HBsAg.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Desnaturalización Proteica / Solventes / Temperatura / Química / Virus de la Hepatitis B / Vacunas contra Hepatitis B / Sulfato de Amonio / Antígenos de Superficie de la Hepatitis B / Concentración de Iones de Hidrógeno Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2010 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Desnaturalización Proteica / Solventes / Temperatura / Química / Virus de la Hepatitis B / Vacunas contra Hepatitis B / Sulfato de Amonio / Antígenos de Superficie de la Hepatitis B / Concentración de Iones de Hidrógeno Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2010 Tipo del documento: Artículo