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Isolation, cultivation and identification of adipose-derived stem cell in bovines / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1645-1651, 2010.
Artículo en Chino | WPRIM | ID: wpr-351553
ABSTRACT
To obtain bovine adipose-derived stem cells (ADSCs), bovine ADSCs were digested in collagenase type I solution. The growth curve of ADSCs was checked by cell counting. Chromosome analysis was checked. The molecular markers of ADSCs were detected with immunofluorescence staining. The morphology of ADSCs was identical to fibroblast like and the cells showed active proliferative ability. Vimentin, CD49d and CD13 antigens were detected, but CD34 antigen was negative. Alkaline phosphatase activity was greater in ADSCs during calcification, and Alizarin Red staining was positive. Lipid droplets were apparent around cells during adipogenesis, and Oil Red-O staining was positive. The results demonstrated that ADSCs could be used as seed cells for tissue engineering due to the simple isolation, differentiation and stable and active growth.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fisiología / Diferenciación Celular / Separación Celular / Células Cultivadas / Tejido Adiposo / Biología Celular / Ingeniería de Tejidos / Células Madre Mesenquimatosas / Métodos Tipo de estudio: Estudio diagnóstico Límite: Animales Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2010 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fisiología / Diferenciación Celular / Separación Celular / Células Cultivadas / Tejido Adiposo / Biología Celular / Ingeniería de Tejidos / Células Madre Mesenquimatosas / Métodos Tipo de estudio: Estudio diagnóstico Límite: Animales Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2010 Tipo del documento: Artículo