Mechanism of DNA transformation based on mineral nanofibers and method improvement / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1379-1384, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-351583
ABSTRACT
Sepiolite--an inexpensive, resourceful, fibrous yet inoffensive mineral--made DNA transformation rapid, simple and efficient but the mechanism for DNA transformation was still unclear. Through RNA competition test, we proposed the different transforming mechanisms from the previous report. Meanwhile, we optimized the transforming method and could transfer a colony stored at 4 degrees C for a month with plasmid through sepiolite fibers. The cells could be transformed well without competent cells preparation or incubation process. In sum, this was a novel potential transforming method, which could be explored further if the chemical method and electroporation could not be used.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Transformación Bacteriana
/
ADN Bacteriano
/
Química
/
Silicatos de Magnesio
/
Electroporación
/
Fibras Minerales
/
Nanofibras
/
Genética
/
Métodos
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2010
Tipo del documento:
Artículo
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