Studies on plantlet regeneration and propagation of Gentiana stramines / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 797-800, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-351792
ABSTRACT
<p><b>OBJECTIVE</b>To provide scientific basis for large scale production by studying the technique of tissue culture of Gentiana stramines.</p><p><b>METHOD</b>Callus was induced from germ-free stem segment of G. stramines on a MS medium supplemented with different hormones.</p><p><b>RESULT</b>The MS medium with 0.5-1.0 mg x L(-1) BA and 0.5 mg x L(-1) NAA was suitable for the induction and proliferation of cluster bads. MS medium with 1.0 mg x L(-1) IAA and 3 mg x L(-1) BA was suitable for the induction of calli. MS medium with 1.0 mg x L(-1) IAA and 2.0-3.0 mg x L(-1) BA was suitable for the subculture of calli. MS medium with 2.0 mg x L(-1) BA and 0.5 mg x L(-1) NAA was suitable for the differentiation of calli.</p><p><b>CONCLUSION</b>Aseptic seeding of G. stramines can be quickly propagated by shoot culture.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Reguladores del Crecimiento de las Plantas
/
Plantas Medicinales
/
Regeneración
/
Tallos de la Planta
/
Medios de Cultivo
/
Gentiana
/
Plantones
/
Técnicas de Cultivo de Tejidos
/
Métodos
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2006
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS